David Samuel Warner

David Samuel Warner

Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine

Humans may sustain a variety of forms of acute central nervous system injury including ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection Laboratories is dedicated to examining the pathophysiology of acute brain and spinal cord injury with particular reference to disease states managed in the perioperative or neurointensive care environments. Rodent recovery models of cerebral ischemia, traumatic brain injury, cardiopulmonary bypass, subarachnoid hemorrhage, spinal cord ischemia, and perinatal hypoxia have been established with requisite control of relevant physiologic variables. Experimental protocols examine the response of brain to these insults and seek to define appropriate therapeutic interventions. Our work examines the role reactive oxygen species in CNS injury with emphasis on how pharmacologic or genetic variants modulate these processes. Effects of altered synthesis of superoxide dismutase and apolipoprotein E are investigated in transgenic/knock out mice. Outcome studies allow definition of efficacy of pharmacologic agents including superoxide dismutase mimetics, PARP-1 inhibitors, hepatocyte growth factor mimetics, recombinant apolipoprotein E and its peptide fragments, SNO-hemoglobin, and anesthetics on histologic and behavioral recovery from ischemic/traumatic insults. Recent focus has been on SUMOylation responses of neural tissue to ischemic stress. Neurochemical, immunohistochemical, molecular biologic, genomic, and proteomic techniques are used to define the mechanistic basis of observations made in outcome studies. Primary neuronal/glial cultures, organotypic hippocampal slices and immortalized transfected human cell lines are used investigate mechanistic interactions between pharmacologic agents and metabolic stresses.

Appointments and Affiliations

  • Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine
  • Professor of Anesthesiology
  • Vice-Chair, Research
  • Chief, Division of Basic Sciences
  • Professor in Neurobiology
  • Professor of Surgery
  • Faculty Network Member of the Duke Institute for Brain Sciences

Contact Information

  • Office Location: 303 Research Dr., 134 Sands Buildign, Durham, NC 27710
  • Office Phone: (919) 684-6633
  • Email Address: david.warner@duke.edu

Education

  • M.D. University of Wisconsin - Madison, 1980

Awards, Honors, and Distinctions

  • Award For Translational Research Mentoring. Duke University School of Medicine. 2015
  • Award for Mentoring Excellence in Research. Foundation for Anesthesia Education and Research (American Society of Anesthesiologists). 2015
  • Distinguished Alumnus Award for Achievement. University of Iowa Carver College of Medicine. 2015
  • Distinguished Service Award. Society for Neurosciences In Anesthesiology and Critical Care. 2011
  • Neuroscience Award. Azzociazione Per La Diffusione E L'Avanzamento Delle Neuroscienze Toscano. 2011
  • Teacher Of The Year. Department of Anesthesiology, DUMC. 2011
  • Excellence in Research. American Society of Anesthesiologists. 2005

Representative Publications

  • Yates, RB; Sheng, H; Sakai, H; Kleven, DT; DeSimone, NA; Stafford-Smith, M; Warner, DS, Lack of evidence for a remote effect of renal ischemia/reperfusion acute kidney injury on outcome from temporary focal cerebral ischemia in the rat., J Cardiothorac Vasc Anesth, vol 27 no. 1 (2013), pp. 71-78 [10.1053/j.jvca.2012.06.012] [abs].
  • Tovmasyan, A; Sheng, H; Weitner, T; Arulpragasam, A; Lu, M; Warner, DS; Vujaskovic, Z; Spasojevic, I; Batinic-Haberle, I, Design, mechanism of action, bioavailability and therapeutic effects of mn porphyrin-based redox modulators., Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre, vol 22 no. 2 (2013), pp. 103-130 [10.1159/000341715] [abs].
  • Topjian, AA; Berg, RA; Bierens, JJLM; Branche, CM; Clark, RS; Friberg, H; Hoedemaekers, CWE; Holzer, M; Katz, LM; Knape, JTA; Kochanek, PM; Nadkarni, V; van der Hoeven, JG; Warner, DS, Brain resuscitation in the drowning victim., Neurocritical Care, vol 17 no. 3 (2012), pp. 441-467 [10.1007/s12028-012-9747-4] [abs].
  • Rajic, Z; Tovmasyan, A; Spasojevic, I; Sheng, H; Lu, M; Li, AM; Gralla, EB; Warner, DS; Benov, L; Batinic-Haberle, I, A new SOD mimic, Mn(III) ortho N-butoxyethylpyridylporphyrin, combines superb potency and lipophilicity with low toxicity., Free Radic Biol Med, vol 52 no. 9 (2012), pp. 1828-1834 [10.1016/j.freeradbiomed.2012.02.006] [abs].
  • Indraswari, F; Wang, H; Lei, B; James, ML; Kernagis, D; Warner, DS; Dawson, HN; Laskowitz, DT, Statins improve outcome in murine models of intracranial hemorrhage and traumatic brain injury: a translational approach., J Neurotrauma, vol 29 no. 7 (2012), pp. 1388-1400 [10.1089/neu.2011.2117] [abs].
  • Sheng, H; Spasojevic, I; Tse, HM; Jung, JY; Hong, J; Zhang, Z; Piganelli, JD; Batinic-Haberle, I; Warner, DS, Neuroprotective efficacy from a lipophilic redox-modulating Mn(III) N-Hexylpyridylporphyrin, MnTnHex-2-PyP: rodent models of ischemic stroke and subarachnoid hemorrhage., Journal of Pharmacology and Experimental Therapeutics, vol 338 no. 3 (2011), pp. 906-916 [10.1124/jpet.110.176701] [abs].
  • Tovmasyan, AG; Rajic, Z; Spasojevic, I; Reboucas, JS; Chen, X; Salvemini, D; Sheng, H; Warner, DS; Benov, L; Batinic-Haberle, I, Methoxy-derivatization of alkyl chains increases the in vivo efficacy of cationic Mn porphyrins. Synthesis, characterization, SOD-like activity, and SOD-deficient E. coli study of meta Mn(III) N-methoxyalkylpyridylporphyrins., Dalton Trans, vol 40 no. 16 (2011), pp. 4111-4121 [10.1039/c0dt01321h] [abs].
  • Sheng, H; Reynolds, JD; Auten, RL; Demchenko, IT; Piantadosi, CA; Stamler, JS; Warner, DS, Pharmacologically augmented S-nitrosylated hemoglobin improves recovery from murine subarachnoid hemorrhage., Stroke; a Journal of Cerebral Circulation, vol 42 no. 2 (2011), pp. 471-476 [10.1161/STROKEAHA.110.600569] [abs].
  • Guinn, NR; McDonagh, DL; Borel, CO; Wright, DR; Zomorodi, AR; Powers, CJ; Warner, DS; Lam, AM; Britz, GW, Adenosine-induced transient asystole for intracranial aneurysm surgery: a retrospective review., J Neurosurg Anesthesiol, vol 23 no. 1 (2011), pp. 35-40 [10.1097/ANA.0b013e3181ef2b11] [abs].
  • Wang, Z; Yang, W; Britz, GW; Lombard, FW; Warner, DS; Sheng, H, Development of a simplified spinal cord ischemia model in mice., J Neurosci Methods, vol 189 no. 2 (2010), pp. 246-251 [10.1016/j.jneumeth.2010.04.003] [abs].
  • Pearlstein, RD; Higuchi, Y; Moldovan, M; Johnson, K; Fukuda, S; Gridley, DS; Crapo, JD; Warner, DS; Slater, JM, Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain., Int J Radiat Biol, vol 86 no. 2 (2010), pp. 145-163 [10.3109/09553000903419965] [abs].
  • Sheng, H; Yang, W; Fukuda, S; Tse, HM; Paschen, W; Johnson, K; Batinic-Haberle, I; Crapo, JD; Pearlstein, RD; Piganelli, J; Warner, DS, Long-term neuroprotection from a potent redox-modulating metalloporphyrin in the rat., Free Radic Biol Med, vol 47 no. 7 (2009), pp. 917-923 [10.1016/j.freeradbiomed.2009.05.039] [abs].
  • Takata, K; Sheng, H; Borel, CO; Laskowitz, DT; Warner, DS; Lombard, FW, Simvastatin treatment duration and cognitive preservation in experimental subarachnoid hemorrhage., J Neurosurg Anesthesiol, vol 21 no. 4 (2009), pp. 326-333 [10.1097/ANA.0b013e3181acfde7] [abs].
  • Mackensen, GB; McDonagh, DL; Warner, DS, Perioperative hypothermia: use and therapeutic implications., J Neurotrauma, vol 26 no. 3 (2009), pp. 342-358 [10.1089/neu.2008.0596] [abs].