John Franklin Rawls
Associate Professor of Molecular Genetics and Microbiology
Research in my lab uses multiple complementary approaches to understand how host-microbe interactions in the intestine regulate digestive physiology and energy balance. First, my lab uses genetic, gnotobiotic, and in vivo imaging approaches to determine how commensal microorganisms (microbiota) interact with vertebrate hosts to regulate their nutrition and immunity, as well as the mechanisms underlying assembly of intestinal microbial communities. We utilize the zebrafish as a host model in which host and microbial cells can be viewed and manipulated a transparent living vertebrate. We have pioneered the use of germ-free or gnotobiotic zebrafish to investigate the roles of microorganisms in vertebrate biology, and we are using these methods to investigate the bacterial signals and responsive host pathways that regulate host immunity, nutrition, and gene expression. Second, my laboratory is utilizing the zebrafish system to investigate mechanisms underlying the formation and function of adipose tissues. We have developed methods for in vivo imaging of zebrafish adipose tissue, and we are currently using these techniques to explore the developmental and environmental processes regulating adipose tissue growth and physiology. In both of these fields, we have effectively used zebrafish and mice to model key aspects of human physiology and pathophysiology, and to gain new insights into underlying mechanisms.
Appointments and Affiliations
- Associate Professor of Molecular Genetics and Microbiology
- Associate Professor in Medicine
- Member of the Duke Cancer Institute
- Office Location: 323A CARL Building Box 3580, 213 Research Drive, Durham, NC 27710
- Office Phone: (919) 613-7212
- Email Address: email@example.com
- Web Pages:
- Ph.D. Washington University, 2001
Awards, Honors, and Distinctions:
- Fellow. American Association for the Advancement of Science. 2016
- Kavli Fellow. Kavli Frontiers of Science Program. 2009
- Pew Scholar in the Biomedical Sciences. Pew Charitable Trust. 2008
- Mentored Research Scientist Development Award. National Institutes of Health. 2006
- Ruth L. Kirschstein Individual National Research Service Award. National Institutes of Health. 2002
- Spencer T. and Ann W. Olin Medical Scientist Fellow. Washington University School of Medicine. 2001
- Victor Hamburger Prize in Developmental Biology. Washington University School of Medicine. 2001
- MGM 593: Research Independent Study
- Davison, JM; Lickwar, CR; Song, L; Breton, G; Crawford, GE; Rawls, JF, Microbiota regulate intestinal epithelial gene expression by suppressing the transcription factor Hepatocyte nuclear factor 4 alpha., Genome research, vol 27 no. 7 (2017), pp. 1195-1206 [10.1101/gr.220111.116] [abs].
- Minchin, JEN; Rawls, JF, A classification system for zebrafish adipose tissues., Disease models & mechanisms, vol 10 no. 6 (2017), pp. 797-809 [10.1242/dmm.025759] [abs].
- Minchin, JEN; Rawls, JF, In vivo imaging and quantification of regional adiposity in zebrafish., Methods in cell biology, vol 138 (2017), pp. 3-27 [10.1016/bs.mcb.2016.11.010] [abs].
- Melancon, E; Gomez De La Torre Canny, S; Sichel, S; Kelly, M; Wiles, TJ; Rawls, JF; Eisen, JS; Guillemin, K, Best practices for germ-free derivation and gnotobiotic zebrafish husbandry., Methods in cell biology, vol 138 (2017), pp. 61-100 [10.1016/bs.mcb.2016.11.005] [abs].
- Bae, S; Mueller, O; Wong, S; Rawls, JF; Valdivia, RH, Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe., Proceedings of the National Academy of Sciences of USA, vol 113 no. 49 (2016), pp. 14127-14132 [10.1073/pnas.1612753113] [abs].