Christina Eleanor Barkauskas

Image of Christina Eleanor Barkauskas

Assistant Professor of Medicine

I am a physician-scientist and stem cell biologist focused on understanding the fundamental mechanisms involved in lung injury and repair. I am particularly interested in determining the critical components of the alveolar epithelial stem cell niche and the cellular crosstalk signals that dictate cell identity and behavior during steady state maintenance and repair after injury and infection. I believe this work is fundamental to better understanding the biology of the human lung and the pathogenesis of idiopathic pulmonary fibrosis (IPF), a disease that is characterized by relentless scar accumulation in the lung with only minimally effective therapy short of lung transplant.

Through my work, I have developed several areas of expertise which include the following: 1) Development and use of mouse models to genetically manipulate and lineage trace specific cell populations; 2) Isolation and characterization of murine lung cell subpopulations by flow cytometry; 3) Development and use of an ex vivo 3-dimensional organoid assay to study the self-renewal and differentiation of murine alveolar epithelial stem cells and their niche components; 4) Isolation and 3-dimensional culture of alveolar epithelial cells from fresh human lung; 5) Development and analysis of models of experimental lung fibrosis.

Appointments and Affiliations

  • Assistant Professor of Medicine
  • Affiliate of the Regeneration Next Initiative

Contact Information:

  • Office Location: Dept of Medicine, Durham, NC 27710
  • Office Phone: (919) 681-6845
  • Email Address: christina.barkauskas@duke.edu

Education:

  • Duke University School of Medicine, 2012
  • Duke University School of Medicine, 2011
  • Duke University School of Medicine, 2009
  • Duke University, 2008
  • Duke University, 2007
  • M.D. Duke University School of Medicine, 2004

Representative Publications:

    • Barkauskas, CE; Chung, M-I; Fioret, B; Gao, X; Katsura, H; Hogan, BLM, Lung organoids: current uses and future promise., Development (Cambridge), vol 144 no. 6 (2017), pp. 986-997 [10.1242/dev.140103] [abs].
    • Wansleeben, C; Barkauskas, CE; Rock, JR; Hogan, BLM, Erratum: Stem cells of the adult lung: Their development and role in homeostasis, regeneration, and disease [WIREs Dev Biol, 2, (2013), 131-148] doi: 10.1002/wdev.58, Wiley Interdisciplinary Reviews: Developmental Biology, vol 4 no. 3 (2015), pp. 311-312 [10.1002/wdev.184] [abs].
    • Fedeles, SV; So, J-S; Shrikhande, A; Lee, SH; Gallagher, A-R; Barkauskas, CE; Somlo, S; Lee, A-H, Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity., Journal of Clinical Investigation, vol 125 no. 5 (2015), pp. 1955-1967 [10.1172/jci78863] [abs].
    • Jain, R; Barkauskas, CE; Takeda, N; Bowie, EJ; Aghajanian, H; Wang, Q; Padmanabhan, A; Manderfield, LJ; Gupta, M; Li, D; Li, L; Trivedi, CM; Hogan, BLM; Epstein, JA, Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung., Nature Communications, vol 6 (2015) [10.1038/ncomms7727] [abs].
    • Alder, JK; Barkauskas, CE; Limjunyawong, N; Stanley, SE; Kembou, F; Tuder, RM; Hogan, BLM; Mitzner, W; Armanios, M, Telomere dysfunction causes alveolar stem cell failure., Proceedings of the National Academy of Sciences of USA, vol 112 no. 16 (2015), pp. 5099-5104 [10.1073/pnas.1504780112] [abs].